Protease Activity Detection
Cut - N - Glow™
Successful detection of Protein Activity
Specific proteases or protease inhibitors or
mapping protease substrate preference in vitro
Cut-N-Glow™ is the first fully biological in vivo protease mapping tool that emits fluorescence. This assay is easily tailored via standard cloning techniques to detect specific proteases or protease inhibitors or to map protease substrate preference in vitro or in vivo. Chemical synthesis is not required and there is no need for co-factors or co-substrates. Additionally, this assay only requires two reagents and both are proteins that can be easily obtained following over-expression of E. coli.