In an impressive demonstration of both technologies, scientists at Confocal.nl stained SK-BR-3 cells with Sandia Biotech's anti-Her2-RFP FluorAbody. Sandia Biotech’s FluorAbodies are unique probes that provide a consistent and cost-effective option to monoclonal antibodies. The molecular architecture of a FluorAbody, consisting of a fluorescent protein linked to an ScFv, results in an intrinsically fluorescent probe with a 1:1 fluorophore to epitope ratio. These distinctive features allow for true quantitation of biomarkers, prevent cross-linking of their targets, and shorten staining protocols by making blocking or secondary antibodies superfluous. Combined, these properties tackle many shortcomings of conjugated, monoclonal antibodies.
4D5-8 single-chain variable fragment (scFv) was used to construct the Anti-Her2-RFP FluorAbody.
Importance: 1:1 Ratio of binding site to Fluorescent Protein
- Better quantitation and reproducible reagent performance compared to fluorochrome-labeled antibodies