Sandia Biotech is proud to announce a strategic partnership with Confocal.nl, the Dutch Microscopy Company. Please join us as we combine our FluorAbody® technology with a game-changing confocal imaging platform that provides super-resolution capability with higher sensitivity than most confocal microscopes. Confocal.nl has advanced the art for super resolution and demonstrated low laser power (see confocal.nl challenge) that enables important biologically relevant applications using spheroids, organoids, and tumoroids. Sandia Biotech and Confocal.nl in partnership with Spirochrome, a Swiss Company, are advancing the art of live cell imaging with no wash, multicolor fluorogenic probes for live cell fluorescence nanoscopy. https://bright-i.nl/home-7/the-challenge/
Timelapse of approximately 1 hour of HUVECs stained for Actin-RFP (red), Mitochondria-GFP (green), and Nucleus-SiR DNA (magenta). Imaged with the Re-scan Confocal Microscope (RCM) on an Olympus IX83 with 1.5NA 100x TIRF objective. Laser power for GFP: 1 microwatt, RFP: 1 microwatt, SiR DNA: 3 microwatts. Sample Courtesy: Philippa Phelp, Amsterdam UMC, location VUmc, Amsterdam.
Trastuzumab (Herceptin™) is a monoclonal antibody (huMAb4D5-8) that targets the Her2 receptor protein and is widely used in immunotherapy of breast cancer.
4D5-8 single-chain variable fragment (scFv) was used to construct the Anti-Her2-RFP FluorAbody.
Importance: 1:1 Ratio of binding site to Fluorescent Protein
- Better quantitation and reproducible reagent performance compared to fluorochrome-labeled antibodies
Human Breast Cancer Cell Line SK-BR-3; ErbB2 Staining with Anti-Her2 RFP FluorAbody®
SK-BR-3 cells were fixed with 4% paraformaldehyde containing DAPI and incubated with anti-Her2-mRFP. Nuclei are blue and ErbB2 (as detected by anti-Her2-RFP) is labeling the cellular membrane (red). Cells were imaged in epifluorescence with TRITC and DAPI filters and a 63x oil objective.
Formalin Fixed and Parafin-Embedded Tissue; Human ErbB2 Staining Shows High Her2 Positive Breast Cancer Tissue
Immunofluorescence staining and detection of Her2 (ErbB2) biomarker in formalin-fixed and parafin-embedded breast tissue from a Her2 positive tissue sample. This result clearly shows that the Anti-Her2 FluorAbody can be used as a diagnostic reagent in breast cancer tissue.
Triple Negative Epithelial Human Breast Cancer Cell Line MDAMB231 Labeled with Anti-EphA2-Cerlulean FluorAbody®
Immunofluorescence analysis of the anti-EphA2-cerulean biomarker. (A) Labeling of the EphA2 receptor with primary and secondary antibody fluorochrome labeled with AF488. (B) Labeling of EphA2 with the anti-EphA2-cerulean FluorAbody. The epithelial human breast cancer cell line, MDAMB231, was stimulated with dimeric ephrinA1-F. The AF488 labeled sample shows a more diffuse staining pattern when compared to the distinct punctate staining pattern shown using the anti-EphA2- cerulean FluorAbody. This result clearly shows that the FluorAbody reagent is superior and provides a more specific staining pattern with more biologically relevant imaging results.
Sandia Biotech Products
Anti-Her2 RFP FluorAbody
SuperFolder GFP Fluorescence in Thermophilus
N-terminus linked ferritin folds correctly and C-terminus folds poorly
Sandia Biotech Application Notes
Long-term imaging with the RCM -Mitochondria timelapse 61 hours
Living mitochondria observed for 61 hours with the RCM add-on. 1 frame was taken every 10 seconds, giving a total of 22.000 frames. Light exposure at sample plane: 1 microwatt.
3D image of mitotic Hela cells imaged with RCM
This 3D reconstruction shows mitotic Hela cells stained for DAPI (blue), Actin (green) and Tubulin (red). Sample courtesy: Nicolas Touret, University of Alberta
