4D5-8 single-chain variable fragment (scFv) was used to construct the Anti-Her2-RFP FluorAbody.
Importance: 1:1 Ratio of binding site to Fluorescent Protein
- Better quantitation and reproducible reagent performance compared to fluorochrome-labeled antibodies
Human Breast Cancer Cell Line SK-BR-3; ErbB2 Staining with Anti-Her2 RFP FluorAbody®
Formalin Fixed and Parafin-Embedded Tissue; Human ErbB2 Staining Shows High Her2 Positive Breast Cancer Tissue
Triple Negative Epithelial Human Breast Cancer Cell Line MDAMB231 Labeled with Anti-EphA2-Cerlulean FluorAbody®
Living mitochondria observed for 61 hours with the RCM add-on. 1 frame was taken every 10 seconds, giving a total of 22.000 frames. Light exposure at sample plane: 1 microwatt.
This 3D reconstruction shows mitotic Hela cells stained for DAPI (blue), Actin (green) and Tubulin (red). Sample courtesy: Nicolas Touret, University of Alberta