In an impressive demonstration of both technologies, scientists at Confocal.nl stained SK-BR-3 cells with Sandia Biotech's anti-Her2-RFP FluorAbody. Sandia Biotech’s FluorAbodies are unique probes that provide a consistent and cost-effective option to monoclonal antibodies. The molecular architecture of a FluorAbody, consisting of a fluorescent protein linked to an ScFv, results in an intrinsically fluorescent probe with a 1:1 fluorophore to epitope ratio. These distinctive features allow for true quantitation of biomarkers, prevent cross-linking of their targets, and shorten staining protocols by making blocking or secondary antibodies superfluous. Combined, these properties tackle many shortcomings of conjugated, monoclonal antibodies.
Human Breast Cancer Cell Line SK-BR-3; ErbB2 Staining with Anti-Her2 RFP FluorAbody
SK-BR-3 cells were fixed with 4% paraformaldehyde containing DAPI and incubated with anti-Her2-mRFP. Nuclei are blue and ErbB2 (as detected by anti-Her2-RFP) is labeling the cellular membrane (red). Cells were imaged in epifluorescence with TRITC and DAPI filters and a 63x oil objective
Formalin Fixed and Parafin-Embedded Tissue; Human ErbB2 Staining Shows High Her2 Positive Breast Cancer Tissue.
Immunofluorescence staining and detection of Her2 (ErbB2) biomarker in formalin-fixed and parafin-embedded breast tissue from a Her2 positive tissue sample. This result clearly shows that the Anti-Her2 FluorAbody can be used as a diagnostic reagent in breast cancer tissue.
Triple Negative Epithelial Human Breast Cancer Cell Line MDAMB231 Labeled with Anti-EphA2-Cerlulean FluorAbody®
Immunofluorescence analysis of the anti-EphA2-cerulean biomarker. (A) Labeling of the EphA2 receptor with primary and secondary antibody fluorochrome labeled with AF488. (B) Labeling of EphA2 with the anti-EphA2-cerulean FluorAbody. The epithelial human breast cancer cell line, MDAMB231, was stimulated with dimeric ephrinA1-F. The AF488 labeled sample shows a more diffuse staining pattern when compared to the distinct punctate staining pattern shown using the anti-EphA2- cerulean FluorAbody.This result clearly shows that the FluorAbody reagent is superior and provides a more specific staining pattern with more biologically relevant imaging results.