In an impressive demonstration of both technologies, scientists at Confocal.nl stained SK-BR-3 cells with Sandia Biotech's anti-Her2-RFP FluorAbody. Sandia Biotech’s FluorAbodies are unique probes that provide a consistent and cost-effective option to monoclonal antibodies. The molecular architecture of a FluorAbody, consisting of a fluorescent protein linked to an ScFv, results in an intrinsically fluorescent probe with a 1:1 fluorophore to epitope ratio. These distinctive features allow for true quantitation of biomarkers, prevent cross-linking of their targets, and shorten staining protocols by making blocking or secondary antibodies superfluous. Combined, these properties tackle many shortcomings of conjugated, monoclonal antibodies.
PRODUCTS
SPLIT-FLUORESCENT PROTEIN
FLUORABODY
GPER
Human Breast Cancer Cell Line SK-BR-3; ErbB2 Staining with Anti-Her2 RFP FluorAbody
SK-BR-3 cells were fixed with 4% paraformaldehyde containing DAPI and incubated with anti-Her2-mRFP. Nuclei are blue and ErbB2 (as detected by anti-Her2-RFP) is labeling the cellular membrane (red). Cells were imaged in epifluorescence with TRITC and DAPI filters and a 63x oil objective
Formalin Fixed and Parafin-Embedded Tissue; Human ErbB2 Staining Shows High Her2 Positive Breast Cancer Tissue.
Immunofluorescence staining and detection of Her2 (ErbB2) biomarker in formalin-fixed and parafin-embedded breast tissue from a Her2 positive tissue sample. This result clearly shows that the Anti-Her2 FluorAbody can be used as a diagnostic reagent in breast cancer tissue.
Triple Negative Epithelial Human Breast Cancer Cell Line MDAMB231 Labeled with Anti-EphA2-Cerlulean FluorAbody®
Immunofluorescence analysis of the anti-EphA2-cerulean biomarker. (A) Labeling of the EphA2 receptor with primary and secondary antibody fluorochrome labeled with AF488. (B) Labeling of EphA2 with the anti-EphA2-cerulean FluorAbody. The epithelial human breast cancer cell line, MDAMB231, was stimulated with dimeric ephrinA1-F. The AF488 labeled sample shows a more diffuse staining pattern when compared to the distinct punctate staining pattern shown using the anti-EphA2- cerulean FluorAbody.This result clearly shows that the FluorAbody reagent is superior and provides a more specific staining pattern with more biologically relevant imaging results.
